DLPNIRNANOEVM: DLPNIRNANOEVM

Hello Kakushik,

Please give us some time to look into this query and we will get back to you.

Thanks,

Kyle

Hello Kyle,

Will be waiting for your response.

Thanks,

Kaushik

Hello Kyle,

Can we have a skype call regarding the above querires?

Thanks and regards

Kaushik

Biofi

Hello Kyle,

As a matter of little urgency,  I request you to get back asap.

Thanks 

Kaushik

Biofi

Hello Kyle,

I am still awaiting your response. Could you kindly connect us through linkedin or any other source for a discussion.

Thanks and regards,

Kaushik

Biofi

Hello Biofi,

Sorry for the delay. One of my colleagues will be looking into this. I will let him know that you are still waiting for a response.

Thanks,

Kyle

Hi Kakushik,

Are you trying to measure glucose in solution with a liquid that is transparent to NIR light? I'm not an expert in measuring glucose, but I would think it would absorb energy at certain wavelengths while being in a solution that transmits those wavelengths. If the scans you are receiving are very noisy and have very high absorbance at all wavelengths, it is likely that your sample measurements are not getting much light into the instrument because your sample is not reflecting much light.. As such, would transmissive sampling be more appropriate for what you are trying to do?

We have done some reflectance measurement of specific liquids that have a high reflectance, like milk which reflects some wavelengths from the milk solids. But I have not heard of that method being used for glucose. Sometimes liquids are also tested in a 'transflectance' mode, where a mirror or diffuse reflective material is placed behind the liquid being measured.

Hello Eric,

Can we please have a call through skype or any other source feasible for you. Actually, we are still facing the issues even with a highly reflective surface placed for the liquid during taking a spectral graph. Kindly, give us us few valuable minutes of you on call so that we can sort this out as soon as possible.

Thanks and regards,

Kaushik

Biofi

Hello Eric,

Also, can you please guide me on how to perform internal diagnostics test on the device. We have pruchased DLPNIR NANO Scan with great hopes and there is no proper response from Texas instruments. Please look into this as we are sitting ducks here with the instrument not giving any proper results.

Thanks and regards,

Kaushik

Biofi

Hello Kaushik,

I am sorry that you are facing difficulty in using DLP NANO Scan EVM for your application. 

Could you please share more details about your application?  What are you trying to and what problem are you trying to solve?

Lets address two key issues first:

1. Is the EVM working correctly and scan results are consistent?

  - you mentioned that  "For reference we are using a highly reflective standard" . Could you please share detail about the standard? 

  - Please  take reference scan using this standard and also scan of a know sample ( it could reflective standard with lower reflection). Please upload detailed CSV file so that I could analyze it and see if there is problem with the EVM.

2. The second important issue is scanning method - The scan method is critical for consistent performance. The following condition will create inconsistent results

   - inconsistent sample presentation

  - Week signal

 - Granular samples or suspended particles in liquid sample will light scattering 

- Temperature and humidity variation

Could you please answer following questions:

 1. How is your sample solid or liquid? 

 2. How is this sample presented for scanning?

Could you please upload CSV file with scan of the sample with two different concentration?

regards,

Vivek

Hello Vivek,

1. Iam uploading the csv file of the spectra to which i have taken a reference scan. the reflectance standard which i have used is obtained from bruker optics. It is a small probe with a total pathlength of 2mm (1mm where the light is passed to and fro, so a total of 2mm).

2. I have dextrose powder which is highly soluble in water. So, I have water as a solvent and dextrose (D-glucose) as solvent. the concentrations made are 25mg/dl, 50mg/dl, 75mg/dl so on till 500 mg/dl.

Sample is put into a flat bottomed circular cuvette, in which the sample is placed and then the probe is immersed in the sample carefully to avoid any air bubbles.

Please find the attached csv files. The zip file contains the data for different glucose concentrations as mentioned above. Also, reference has been taken for the samples. Kindly, do the needful.

Also, I request you to kindly connect through any online call. That would really be appreciated alot.

Thanks and regards,

kaushik

BiofiData - Different Concentrations.zip

Hi Kaushik,

I reviewed the uploaded CSV files and especially compared the sample signal value in Column D of the file. Following are my observation:

1. Consistency  of data value of repeat measure - They seems to be consistent across multiple scans for same sample.  The variation in value is with in acceptable range .  

  I could not find a case of non repeatable scan value of for same concentration. Could you please . upload CSV file with this problem and high light where (what wavelength) you see this problem.  I believe the EVM is workign correctly. It is likely scanning procedure or system issue.

2. You wrote - "As per theory, absorbance increases with increase in concentration."

I reviewed scan data for across concentration. I noticed following issue:

 - Scan data for 125mgdl concentration is not correct. The reference value (column C) in  CVS file is much lower than similar value for other concentration.

This lead me to question, how are you taking reference value? In this case , the reference is are lower than the scan sample signal value. This is a measurement procedure error because reference scan and sample scan are done separately.

Could you please post pictures  of your set-up while taking a reference and also while scanning a sample? Please also provide me a link to information on probe you are using?

Online call - I am sorry ,  we don't support calls.

If you like, I will be happy to take this discussion off-line and you can share details of your project.

regards,

Vivek

Hello Vivek,

I have attached a detailed presentation with Biofi setup for your understand on How we are taking the scans.

Also, we can take the discussion offline and discuss on this issue. Can you please let me know how to contact you offline.

Thanks 

KaushikBiofi_TI_CASE_DLP_NANOSCAN.pdf

Hi Kaushik,

Thank you for detailed picture.  This does help me understand your system.

I do have few follow-up question.

I will take this discussion off-lien so that we can have open and focused discussion.

Please accept my request for to connect through the system.

I will close this thread.

regards,

Vivek