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DLPNIRNANOEVM: Can detect proteins

Intellectual 2110 points

Replies: 7

Views: 107

Part Number: DLPNIRNANOEVM

Hi Team,

We receive an inquiry from a customer if the DLPNIRNANOEVM can detect proteins. 

(1) Can it detect protein?

(2) If so, what is the limit (PPM)?

(3) Can it be employed with saliva?

(4) How to subtract background?

(5) Can we access raw data?

(6) If I want to use it with machine learning platform, does TI help us with the analysis protocol?

Regards,

Danilo

  • Danilo,

    Can you elaborate on your application in a bit more detail? What kind of proteins are you looking to analyze? What kinds of concentrations?

    You also mentioned analysis of data collected by the NIRscan Nano. Please explain in more detail what kind of assistance you are looking for.

    Regards,

    Philippe Dollo

    If a post answers your question, please click the "Verify Answer" button.

  • In reply to Philippe Dollo:

    Hi Philippe,

    Let me rephrase the questions little bit.

    1) Is there any chemometrics or data mining support available for scans?

    2) The proteins that I am referring to are regular antibodies. One example can be the pregnancy strips.

    3) What is the detection limit?

    4) What is easier to analyze- liquids or dry powder?

    Suman

  • In reply to Suman Sinha Ray:

    Suman,

    Once you have taken a scan, you can export the scan data to .CSV format and perform data processing on it as desired. See section 3.1.2 of the EVM user's guide: http://www.ti.com/lit/ug/dlpu030g/dlpu030g.pdf

    I shall loop in the team to comment on the use case you are referring to.

    Regards,

    Philippe Dollo

    If a post answers your question, please click the "Verify Answer" button.

  • Hi Danilo,

    For your Question 5 & 6:

    (5) Can we access raw data?

    Hirak> Yes, we can access raw intensity data in the excel file that is created after each scan.

    (6)  If I want to use it with machine learning platform, does TI help us with the analysis protocol?

    Hirak> Kindly elaborate more what do you mean by "Analysis Protocol"? In general, TI provides you support regarding usage of NIRScan Nano, any machine learning algorithm/framework you plan to use you have to implement yourself. 

    Also, could you kindly elaborate what do you mean by "Background" in Q4?

    Kindly wait for sometime, we will be answering the rest of the questions soon. Thanks for your patience!

    Regards,

    Hirak.

  • In reply to Hirak Biswas:

    Hello Suman,

    Thank you for your patience. Please find response to your various questions:

    First post -

    1) Can it detect protein?

    TI response - It will depend on your system design, measurement procedure and analytic software. The EVM has 6000:1 SNR and spectral resolution is 10 nm. The critical factor for system  design are :

       a. Consistent and repeatable presentation of sample, minimize variation.

       b. Period white reference to minimize impact of aging, Also minimize impact of humidity and temperature variation on lamp output.

       c. Use of good analytic tools and model.  

    (2) If so, what is the limit (PPM)?

    TI response - It is an evaluation module. The PPM limit will depend on the system as mentioned in previous question.

    (3) Can it be employed with saliva?

    TI response - You have to run your own experiment and explore if there is good signature.

    (4) How to subtract background?

    TI response - You may want to chemomatric  analytic software for this purpose.  Please reach out to our extend echo system partners like Analyze IQ, Saggito and Camo. They may be able to help you on this topic.

    http://www.ti.com/dlp-chip/advanced-light-control/optics-electronics/extended-ecosystem.html

    (5) Can we access raw data?

     TI response - Yes

    (6) If I want to use it with machine learning platform, does TI help us with the analysis protocol?

    TI response - TI does not have such tools. Chemotric software and tool are available from our echo system partners. Please contact them directly.

    Second post -

    1) Is there any chemometrics or data mining support available for scans?

    TI response - Please contact extend eco system partners.

    2) The proteins that I am referring to are regular antibodies. One example can be the pregnancy strips.

    3) What is the detection limit?

    TI response- same as before ; it is system dependent.

    4) What is easier to analyze- liquids or dry powder?

    TI response- From my personal experience, I got better result with liquid using a transmissive head.

    regards,

    Vivek

  • In reply to Vivek Thakur:

    Thank you for your response. What is a transmissive head?

  • In reply to Suman Sinha Ray:

    Please reffer to following pages on our Eco system partners. They have transmisive head for DLP NIRScan nano.

     

    1. http://www.inno-spectra.com/en/product

    https://www.optecks.com/Portal/index.php/products-root/spectrometers/spectrometer-with-trasmissive-module

    regards,

    Vivek