Hello everybody
I have a DLP NIRscan nano EVM purchased from Texas Instruments.
I made some scans with solutions. My goal is to get a quantitative analysis. My solutions are sucrose dissolved in h2o by different concentrations (1, 2, 3, 4, 5, 10, 15%).
Scanning first h2o (in trasmission mode, I purchased from Optiks the transmissive head) and then the solutions I get the spectra. I'm referring to a scientific article where the researcher did the same thing.
here the link of scientific article: link.springer.com/.../s12596-012-0102-0
Here is the issue: scanning the solutions, increasing the concentration of sucrose in water, I don't get a coherence. In fact increasing sucrose concentration the absorbance peaks should decrease in intensity (like described in paper) but I get a strange effect: increasing the sucrose concentration some peaks are decreased, other ones increased and other ones decreased but in a non-linear way.
My question is: is it possible that the instrument could change detector's sensitivity during a scan (maybe because of different light intensity received)? Is there some electronic control inside the instrument that manage the mathematical values of the spectra in some way?
I have to know this because I'm pretty sure that I'm not making mistakes in measurement setup, nor in cleaning the cuvette.
Hope you could give me a response soon
Thank you