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AFE4400: problems with the EVM

Part Number: AFE4400

hi,

a customer of mines is working on AFE4400.

They are using the EVM to create new calibration.

They have followed the instructions in http://www.ti.com/lit/ug/slau480c/slau480c.pdf,  however after any AFE44x0SPO2EVM GUI initialization, they got the error message in attachment.

So they can only work in SIMULATION mode, and cannot enter in CONNECTED mode.

Can you provide me with any suggestions about root cause and/or how to solve this problem?

Thanks a lot in advance

KR

Vincenzo