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LM3478: My LM3478 design doesn't work very well.

Part Number: LM3478

This is my circuit diagram, and this is my pcb lay out.
I encountered some problems,sometimes I can get HV about 150V.but if I touch some part of this PCB .it doesn't work at all .I can only get the power voltage. I remove all the electronic component,and then weld them on the PCB without change anythings.It works again. I feel so strange. 
And then I make another PCB. it doesnt work at all. 
 It is a part of one PCB. It uses the same schematic of the first one.
THX for TI'ers help.