Other Parts Discussed in Thread: UNIFLASH,
Tool/software:
Is there a command line tool command to get a hex file checksum, which we get in Uniflash when we browse the file
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Got it. On my end, it is showing MD5 instead (using version Uniflash version 9.2.0.5300).

Which version of Uniflash are you using?
Can you try opening an .hex file generated from an out-of-box SDK example?
I've uploaded one here for reference: https://e2e.ti.com/cfs-file/__key/communityserver-discussions-components-files/538/empty_5F00_LP_5F00_EM_5F00_CC2340R5_5F00_freertos_5F00_ticlang.hex
i need to calculate checksum any idea how it is calculated, any dlslite command to get checksum?
I could not find an option in Uniflash CLI (dslite) for that.
This post shows a way to do it in Python, it could be adjusted to print out specifically the calculated CRC or be read from the generated/updated file: (+) CC2340R5: CRC bytes in CC2340's CCFG - Bluetooth forum - Bluetooth®︎ - TI E2E support forums