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Nick,
It does seem you have checked for all the right things, the v1 is the only version of the GUI, we have had some issues with the GUI on different machines, however most have been linked to the regional language settings. Few things to keep in mind when using this GUI are
1. If you use the sliders, don't keep the left mouse clicked and vary the speed. Just vary it quickly to the new position and release the button. When clicked the GUI constantly tries to update the values which may cause the connection to fail.
2. We only tested the GUI with XP and did not use Windows 7, so i am not sure if this could be causing any problems.
3. GUI does require .Net framework installed on the machine, did you check if you have an updated version of the .Net
4. The Gui is constructed to be connected from a power start, so i will recommend powering off the board completely once the connection is lost and then reconnecting.
5. I would also recommend that you start looking at the CCS projects and use them to start spinning the motors in the meanwhile. The GUI is just meant for a visual demo and i will not recommend spending a lot of time getting it working on your machine.
Also just as a note for future kits we are moving to a more robust GUI development platform, to avoid such situations.
I apologize for the inconvenience.
Regards
Manish Bhardwaj