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The following figure illustrates the relative light absorption spectra of oxygenated and deoxygenated haemoglobin with an overlay of the red (660nm) and infrared (900nm) light spectra emitted by the LEDs. The dashed purple line corresponds to a blood mixture that is near 50% oxygen saturation.
Reference: “A technology overview of the Nellcor OxiMax pulse oximery system,” Nellcor Puritan Bennet Inc., 2003
Oxygenated Haemoglobin absorbs less red light than infrared (IR) light and so the blood volume at high saturation has less impact on the detected red signal than on the IR signal. That is, the AC component of the Red Signal is smaller than that of the IR signal.
Deoxygenated Haemoglobin absorbs less IR light than red light and so the blood volume at low saturation has less impact on the detected IR signal than on the red signal. That is, the AC component of the IR signal is smaller than that of the red signal.
The below figure illustrated the red and IR light signals at high and low oxygen saturation. At high saturation, the red 'pulse amplitude' (AC/DC) is smaller than the IR pulse amplitude.
Reference: “A technology overview of the Nellcor OxiMax pulse oximery system,” Nellcor Puritan Bennet Inc., 2003
SpO2 estimation is based on computing the “ratio of ratios” or Pulse Modulation ratio R. In the context of Red and IR LEDs it is defined as:
where the AC component is defined as a peak-to-peak distance of the heart beat signal, and DC component is defined as the middle value between the min and max peaks as demonstrated in the previous figure.
The below figure illustrates the relationship between R and SpO2 that follows a smooth curve that serves as the sensor calibration curve.
Reference: “A technology overview of the Nellcor OxiMax pulse oximery system,” Nellcor Puritan Bennet Inc., 2003
Since the light absorption of the oxygenated and deoxygenated haemoglobin is significantly wavelength-dependent, the relationship between R and SpO2 strongly depends on the specific emission characteristics of the sensor’s LEDs. Accurate measurements of saturation requires the sensor's calibration curve.
hi,
I can't see the picture ,can you send the picture to me ,289016861@qq.com
thank you
Hello Wei,
You should be able to see the pictures when you log in to your E2E account.
Best Regards
Praveen.
Hi,Praveen,
I have logined ,but still can't see the picture. When I click on the picture ,it prompt me that I don't have the permission.
I have search several days on the internet to solve the promblem how to use the afe4400 to calculate the SP02 and this is the only resource that I think can help me a lot. Can you change the permission of the picture or send it by mail to me. I really appreciate it very much.
Best Regards
wei
Hello Wei,
I have sent the article to your email address.
Regards
Praveen.
Hello Praveen,
I have the same problem: even logged in I cannot see the picture (error 403). Can you post a link to the article or is there a way I could get my hands on it?
Regards,
Mathieu
Mathieu,
What is your email address? We could possibly send you a copy through your email.
Hello Amy,
Yep that's right, with an email address it would be easier :)
Mine is: mathieu.hingant@pera.com
Regards,
Mathieu
Amy,
I recently purchased the evaluation kit for the AFE4490 and I'm looking for information on how to calculate the SpO2 and HR values. I know the GUI software that is provided on the kits webpage handles this information in real time, but I'm in need of saving out this information as well. My email is weinle.3@wright.edu. Any help you could offer is greatly appreciated.
Nathan,
I just sent the article mentioned here to you email. That should provide you with some good information about SpO2 calculation and measurement.
Amy,
I am having the same issues trying to view the images. Can you send me some documentation to help me calculate the Pulse-Ox as well for the AFE4490. My email is keatkins42@students.tntech.edu.
Hi Sir,
Iam a Btech student working on Pulse Oximeter. The thread here looks amazing. The image is not visible to me. Kindly send the details to gouthamp@ieee.org
Hello, Can you send me some documentation to help me calculate the SPO2 with using AFE4490. My email is sergey.buljonov@itv.ru
Hello Sergey,
I have sent the document to your email address.
Regards
Praveen.
Greetings
Could your share me this information as well? my email is erwin.lopez@tee.com.co
Here's yet another request for the SpO2 calculation info. My email address is:
h2008f@gmail.com
Thanks!
I hope you can help me with this problem. It seems that the evaluation board is somehow switching sampling frequencies in the middle of a serial read. I am using the serial communication protocol outlined in the available PDF for the AFE44X0 modules. I am simply trying to read 2048 samples of the ADC channels and then plot those samples for channel 1. The result looks like this:
Hello Nathan,
Could you provide more details about your setup, AFE configuration settings?
Is this issue repeatable?
Can you use the PC application GUI and check if you are noticing this issue with your AFE register settings?
Regards.
The issue is definitely repeatable. I'm simply using Matlab and opening the serial port to the AFE and then sending the ADC read command with the argument to request 2048 samples. The issue is present on a range of baud rates from 9600-115200. I've used the PC application and have not had any similar issues.
hi .. i cant see the pictures that you r using to explain... cn u send it to my email
my email id is sankar27.sree@gmail.com
Nathan,
Below is the message communication format for AFE4400SPO2EVM.
2072.Message Communication Protocol - AFE44x0SPO2EVM.pdf
Have you seen this? This might help with some of your matlab debug.
Hello,
I am working on pulse oximeter design using AFE4400. Could you please send SpO2 estimation documents to my id: rahul.jain.bme09@iitbhu.ac.in.
Thanks
Rahul,
If you cannot see the pictures on this original post http://e2e.ti.com/support/applications/high_reliability/f/30/t/279797.aspx there is a link at the bottom of the post to the pdf file. I have also included the link to the pdf here.
I am facing the same problem.. were you able to fix it somehow.
If yes then plz provide me the way..
Atul,
Did you click on the link to the pdf file? You should be able to view the pictures in the file.
Hi Amy,
I have the AFE4490SPO2EVM board . The software provides me values for LED (Red) , LED(IR) , LED(Red)-LED(ambient Red) and LED(IR) - LED(ambient IR). Since I am new to this field, can you please guide me through the calculation to obtain the SPO2 value. I would also like to know if I can perform the calculations through the software or I need to manually calculate it after obtaining the values from the LEDs.
Thank you and waiting for your reply.
Regards.
Santosh
Santosh,
Please see the posts below as they may be useful to you:
e2e.ti.com/.../321205
e2e.ti.com/.../350487
Also see the information below:
he following figure illustrates the relative light absorption spectra of oxygenated and deoxygenated haemoglobin with an overlay of the red (660nm) and infrared (900nm) light spectra emitted by the LEDs. The dashed purple line corresponds to a blood mixture that is near 50% oxygen saturation.
Reference: “A technology overview of the Nellcor OxiMax pulse oximery system,” Nellcor Puritan Bennet Inc., 2003
Oxygenated Haemoglobin absorbs less red light than infrared (IR) light and so the blood volume at high saturation has less impact on the detected red signal than on the IR signal. That is, the AC component of the Red Signal is smaller than that of the IR signal.
Deoxygenated Haemoglobin absorbs less IR light than red light and so the blood volume at low saturation has less impact on the detected IR signal than on the red signal. That is, the AC component of the IR signal is smaller than that of the red signal.
The below figure illustrated the red and IR light signals at high and low oxygen saturation. At high saturation, the red 'pulse amplitude' (AC/DC) is smaller than the IR pulse amplitude.
Reference: “A technology overview of the Nellcor OxiMax pulse oximery system,” Nellcor Puritan Bennet Inc., 2003
SpO2 estimation is based on computing the “ratio of ratios” or Pulse Modulation ratio R. In the context of Red and IR LEDs it is defined as:
where the AC component is defined as a peak-to-peak distance of the heart beat signal, and DC component is defined as the middle value between the min and max peaks as demonstrated in the previous figure.
The below figure illustrates the relationship between R and SpO2 that follows a smooth curve that serves as the sensor calibration curve.
Since the light absorption of the oxygenated and deoxygenated haemoglobin is significantly wavelength-dependent, the relationship between R and SpO2 strongly depends on the specific emission characteristics of the sensor’s LEDs. Accurate measurements of saturation requires the sensor's calibration curve.