Part Number: TCA39306
From spec
Switch Configuration Vref1=Vref2

Translation Configuration Vcc2 >= Vref1 + 0.7 V

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Part Number: TCA39306
From spec
Switch Configuration Vref1=Vref2

Translation Configuration Vcc2 >= Vref1 + 0.7 V

1. Yes.
2. This particular circuit is needed to regulate the EN voltage to Vth above Vref1. (This clamps all output voltages to Vref1, i.e., it prevents higher voltage from showing up at the low-voltage side, and it allows to generate output signals at Vref1 even without a pull-up to Vref1.)
3. The resistor is needed to limit the current that flows into Vref2 and out of Vref1, and to have a voltage drop so that EN is lower than 3.3 V. Note that this means that the two supplies are not isolated from each other; to disable the device, you would have to pull EN low.