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RM44L920: SPI initialisation not working after flashing

Part Number: RM44L920

I am SPI communication for Accelerometer while i debug the program its calibrating but when flash the program and resetting the power the SPI is not initialising , i am sending data over CAN and observing using esp32 CAN,accelerometer data is not calibrating.So my question is that what happens when we debug the program v flashing the program and how it affects the SPI initialistation.