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MSP430FR6043: doubt in meter constant

Part Number: MSP430FR6043

Hello forum,

i had tried for adc capture with and without airflow using MSP430FR6043 for 200khz where i got adc capture (with airflow) with noise so i tried to neglect noise for got an perfect adc capture.so i planned for calculate meter constant,delta tof,absolute tof ...pls help me to find out absolute TOF hence the formula used is 

In here how to find T thresh ups and T thresh dns and T offset ......help me to find mannually abstof as well as to find T12 and T21 because i didnt get the perfect value for C and V ,volume where it is used in formula to find T12 .

pls give me a suggestion for whether it is correct procedure of calculating manually or adjusting the parameter in GUI...how can i deal with that???

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