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DS80PCI402: Application doesn't work

Part Number: DS80PCI402


I have design a Extender Board by using DS80PCI402 for M.2 SSD.

And use the Pin Mode for DS80PCI402. The Problem was it did work properly all the time.

In the beginning , i suspect there were a layout mistake, so i use a spare board then connect TX & RX by a Jumper wire, It can work properly. Means the layout may not a root cause to make this happen.(Jumper  Pin1 to Pin 45,Pin2 to Pin 44  and Pin 10 to Pin.35 , Pin11 to Pin36 )

Does anything wrong to cause this kind of issue? Setting or Strapping value?

AMT18033-0710.pdf