DS90UB954-Q1: Does it work when connecting two 954 and two 953 to make a long chain?

This connection is OK.
I assume you are going to replicate the first 953 input to a CPU and another device?
The red I2C lines need to be connected by trace/wire.

The slave address and slave Alisa need to be configured for each 953 and 954 in the device register.

Best Regards,
Charley Cai

Dear Charley Cai，

    Thank you very much for the replying.

    Yes, you are right. The bypass is to get a replicated video data from the camera sensor for other devices, and those devices would only receive video data without trying to control the camera sensor.

    The camera sensor would be controlled by the CPU through the red i2c bus in the figure. The red i2c lines are traces on printed circuit boards.

    The CPU here might be replaced by an image processor, and it would regularly write to or read from the remote camera sensor through i2c bus during receiving the image stream.  Because the i2c transactions will pass across 2 954-953 chains, and I want the video to be 30 frames per second, should the i2c timing be a problem here?

Best regards,

Tang Yaosheng