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Hello Everyone
For a university class project on biomedical instrumentation, I constructed the following circuit to measure electromyogram signals from the forearm muscles, namely the flexor digitorum superficialis muscle. I want to do an experiment to relate hand grip strength to electromyogram signals measured from the forearm muscles. However this circuit gives inconsistent results; it sometimes measures EMG signals very well, at other times there is allot of noise as shown in the figures in the attachment.
In this circuit schematic U1 is a Burr Brown INA 128 instrumentation amplifier with gain = 20. U2 is an active unity gain first order high pass filter with 50Hz cut off frequency constructed using Texas Instruments OPA2604 op amp. U3 is a low pass filter with cut off frequency of 500Hz. This low pass filter also amplifies the EMG signal, the gain of the amplification is set by R3/R4 = 165. Therefore the total gain of this circuit is 20x165 = 3300. All circuits are powered by +9V/-9V from batteries and each IC has a 0.1 microfarad ceramic capacitor.
Could you all please suggest any improvements I can add to this circuit to solve this noise problem and get consistent EMG recordings? I have read about the usage of the UAF42 as a 50Hz notch filter (Im from Malaysia where power line interference is 50Hz instead of 60Hz). If I add a notch filter, should I put it after the band pass filter or before the band pass filter? Should I replace the simple first order high pass and low pass filters with Sallen Key filter topology?
I also have another question regarding safety. It is common to put a series protection resistor (around 10K to 100K) between each of the recording electrodes and instrumentation amplifier inputs. Another protection resistor (around 1 Megaohm) is usually placed between the ground(elbow) electrode and ground of the EMG circuit. I have tried doing this but these resistors tend to be a cause for noise. Will it be okay to use parallel diodes at the instrumentation amplifier inputs (instead of protection resistors) and do away with the 1 megaohm resistor between my elbow and ground?