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INA826: INA826 Vout Issue

Part Number: INA826

Hello,

About the INA826 with the single power supply application as following circuit.

If using single supply, REF 2.5V is needed, correct ?

Why is the wrong Vout by the measure 1 & 3 ? Vout is corrct with measure 2, but REF is connect to GND ? Could you please help to check for this ? Thank you.